# Assessing Heterogeneity of Treatment Effect, Estimating Patient-Specific Efficacy, and Studying Variation in Odds ratios, Risk Ratios, and Risk Differences

RCT
generalizability
medicine
metrics
personalized-medicine
prediction
subgroup
accuracy-score
2019
This article shows an example formally testing for heterogeneity of treatment effect in the GUSTO-I trial, shows how to use penalized estimation to obtain patient-specific efficacy, and studies variation across patients in three measures of treatment effect.
Author
Affiliation

Vanderbilt University
School of Medicine
Department of Biostatistics

Published

March 25, 2019

Modified

June 7, 2024

## Background

Heterogeneity of treatment effect (HTE), better called differential treatment effect, is variation in a measure of treatment effect on a scale for which it is mathematically possible that such variation be absent even if the treatment has a nonzero effect. The most commonly used scales for measuring HTE are

• the original scale for continuous response variables that are properly transformed, in which case the treatment effect is the (adjusted) difference in means
• odds ratios, for binary or ordinal outcomes
• hazard ratios, for time-to-event outcomes

Define

• $$Y$$: outcome variable
• $$X$$: a vector of baseline patient characteristics
• $$T$$: treatment, with 1=new treatment and 0=standard treatment
• $$F(y | X, T) = \Pr(Y \leq y | X, T)$$: cumulative distribution function of $$Y$$ for a given $$X$$ and $$T$$

Whenever at least some of the $$X$$ covariates relate to $$Y$$ there will be within-treatment patient outcome variability due to these $$X$$s. When $$Y$$ is a binary 0/1 response, only $$F(0 | X, T)$$ is relevant and this is one minus the probability of an event ($$Y=1$$).

Let $$g$$ be a one-to-one monotonic function that maps $$[0, 1]$$ into $$[-\infty, \infty]$$, i.e., $$g$$ transforms the restricted $$[0, 1]$$ range into an unrestricted range. Some typical choices of $$g$$ are as follows:

• linear model: $$g(u) = \Phi^{-1}(u)$$ = normal inverse transformation
• binary logistic model and proportional odds ordinal logistic model: $$g(u) = \text{logit}(u) = \log\frac{u}{1-u}$$ = inverse logistic transform
• binary or ordinal probit model: $$g(u) = \Phi^{-1}(u)$$
• Cox proportional hazards model: $$g(u) = \log(-\log(1 - u))$$ (log cumulative hazard)

$$g$$ is chosen so that on the $$g(F(Y))$$ scale it is possible that $$g(F(y | X, T=1)) - g(F(y | X, T=0))$$ is independent of $$X$$ where there is a nonzero effect of $$T$$. Dependency of that difference on $$X$$ means there is an $$X \times T$$ interaction on the $$g$$ scale. So on the $$g$$ scale it is possible that no $$T$$ interactions exist, i.e., interactions are surprising.

When $$g(u) = \Phi^{-1}(u)$$ for continuous $$Y$$ having a normal distribution with constant variance, the treatment effect is a difference in mean $$Y$$: $$E(Y | X, T=1) - E(Y | X, T=0)$$ which is possible to be independent of $$X$$, i.e., it is possible that $$X \times T$$ interactions are absent so that HTE is zero.

When $$g(u) = u$$ for a binary $$Y$$, we are computing differences in absolute risk. When there is any treatment effect these $$F(0 | X, T=1) - F(0 | X, T=0)$$ differences must be a function of $$X$$ just because of risk magnification, i.e., the differences must “slow down” as one approaches a baseline risk $$F(0 | X, T=0)$$ near 0 or 1. So HTE on the untransformed probability scale will always exist and can be traced merely to generalized baseline risk. Therefore to prevent a pure statistical phenonemom from being labeled as HTE, we need to assess HTE on an unlimited $$g$$ scale.

One possible way to choose $$g$$ is to find the monotonic function meeting the mapping criteria that minimizes the importance of all within $$X$$ and $$X \times T$$ interaction effects. In a wide variety of clinical studies with binary $$Y$$, the logit transformation tends to create the most additive effects and so is an excellent candidate for assessing HTE. That doesn’t mean that the logistic model will fit every situation and that odds ratios are always a magical basis for assessing HTE, but from a pragmatic standpoint the fit of logistic models is very competitive with other unrestricted range link functions $$g$$.

How do we actually quantify HTE? There are two major ways:

• Estimating double differences. For simplicity suppose there is only one baseline covariate, and consider two of its values: $$a, b$$. Compute the differential (interaction) treatment effect
$$F(y | X=a, T=1) - F(y | x=a, T=0) - [F(y | X=b, T=1) - F(y | X=b, T=0)]$$.
• Compute a global measure of differential treatment effect over the entire sample’s covariate distributions. Some possible measures are
• deviance (-2 log-likelihood) that is due to the combined effects of all interaction effects, i.e., compute the model likelihood ratio $$\chi^2$$ statistic with and without all the $$T$$ interactions and take their difference
• fraction of model $$\chi^2$$ due to interactions
• adjusted pseudo partial $$R^2$$ for the combined interaction terms
• AIC, assessing whether inclusion of differential effects makes predictions of future outcomes better than ignoring them

The last idea needs to be considered more frequently, as it estimates likely out-of-sample performance of a more complex model that allows for treatment interactions. Unless the effective sample size is huge, it is often the case that pretending the HTE is absent will result in better (lower mean squared error) $$X$$-specific treatment effect estimates than allowing HTE to be present and having to estimate hard-to-estimate interaction effects. The adjusted pseudo $$R^2$$ approach provides an in-sample unbiased estimate of the explanatary ability of differential treatment effects, accounting for overfitting.

Even though the more complex hazard ratio seems to be well accepted as a summary measure of treatment effect in a time-to-event randomized clinical trial (RCT), there is still a good deal of resistence to odds ratios (OR) from some clinical researchers. This resistence is difficult to understand, although it is clear that ORs are more difficult to understand than risk ratios (RR) or absolute risk reduction (ARR; risk difference). The reasons for choosing ORs are:

• ORs come directly from logistic models, and logistic models are as likely as any model to fit patterns leading to binary responses. This is primarily because the logistic model places no restrictions on the regression coefficients.
• ORs are capable of being constant over a range of baseline risk all the way from 0 to 1.0.
• In the multitude of forest plots present in journal articles depicting RCT results, the constancy of ORs over patient types is impressive.
• Unlike RRs, ORs are invariant to the choice of the “event” vs. the “no event”. If you interchange event and non-event you would get the reciprocal of the original OR, but the RR would change arbitrarily.

RR and ARR are not capable of being constant. For example, a risk factor that doubles the risk of an outcome can only apply to a patient having a baseline risk of 0.5 or less, otherwise the risk with the risk factor would exceed 1.0. A treatment that lowers the risk of an outcome of 0.04 cannot apply to a patient with a baseline risk of 0.02.

• a rigorous test for HTE in the frequentist domain is done for an actual large RCT with a binary endpoint
• the difficulty in estimating and testing interaction effects
• the OR varies over patient types if one allows all the baseline variables to interact with treatment, whether the interactions are “significant” or not
• RR varies over patient types
• ARR varies over patient types

Establishing the existence of differential treatment effects in RCTs is difficult because RCTs are typically sized just large enough to detect an overall average treatment effect. In the ideal situation for showing evidence of HTE, a potentially interacting binary covariate yields optimum precision and power when the covariate has equal sample sizes for its two values. Even then, the precision of the interaction effect (a double difference) is four times as bad as the precision of the overall treatment effect. And as Andrew Gelman has discussed, the power is as if the sample size is divided by 16 because one typically wants to detect a differential effect that is only half as large as the overall detectable treatment effect. A related example will be shown below.

It is important to note that assessing treatment effect in an isolated subgroup defined by a categorical covariate does not establish HTE and results in unreliable estimates. Differential treatment effect must be demonstrated.

## The GUSTO-I Study and Basic Outcome Model

The GUSTO-I Study was a 40,000 patient international trial comparing four treatment arms for 30-day mortality following acute myocardial infarction: streptokinase (SK) with subcutaneous heparin, SK with intravenous heparin, accelerated dosing of tissue plasminogen activator (t-PA) with intravenous heparin, and SK combined with t-PA and intravenous heparin. The covariate-adjusted analysis that forms the basis for this article was done by Steyerberg, who replaced a limited number of missing covariate values with singly imputed values. We will be primarily comparing the accelerated t-PA arm (n=10,320) with the two combined SK arms that did not involve t-PA (n=20,162).

Code
require(Hmisc)
mu <- markupSpecs$html # in Hmisc load(url('http://hbiostat.org/data/repo/gusto.rda')) gusto <- upData(gusto, keep=Cs(day30, tx, age, Killip, sysbp, pulse, pmi, miloc)) Input object size: 5241552 bytes; 29 variables 40830 observations Kept variables day30,tx,age,Killip,sysbp,pulse,pmi,miloc New object size: 1476752 bytes; 8 variables 40830 observations Code html(describe(gusto), scroll=FALSE) gusto Descriptives gusto 8 Variables 40830 Observations day30 nmissingdistinctInfoSumMeanGmd 40830020.19528510.069830.1299 Killip: Killip Class nmissingdistinct 4083004  Value I II III IV Frequency 34825 5141 551 313 Proportion 0.853 0.126 0.013 0.008  age nmissingdistinctInfoMeanGmd.05.10.25.50.75.90.95 4083005575160.913.6240.8944.6952.0961.5269.8676.2579.45 lowest : 19.027 19.031 20.289 20.781 20.969 , highest: 92.953 95 96.547 108 110 pulse: Heart Rate beats/min nmissingdistinctInfoMeanGmd.05.10.25.50.75.90.95 4083001660.99975.4119.55 50 55 62 73 86 98107 lowest : 0 1 6 8 9 , highest: 200 205 210 220 246 sysbp: Systolic Blood Pressure mmHg nmissingdistinctInfoMeanGmd.05.10.25.50.75.90.95 4083002090.99912926.56 92100112130144160170 lowest : 0 30 32 36 40 , highest: 270 274 275 276 280 miloc: MI Location nmissingdistinct 4083003  Value Inferior Other Anterior Frequency 23495 1435 15900 Proportion 0.575 0.035 0.389  pmi: Previous MI nmissingdistinct 4083002  Value no yes Frequency 34104 6726 Proportion 0.835 0.165  tx: Tx in 3 groups nmissingdistinct 4083003  Value SK+tPA SK tPA Frequency 10320 20162 10348 Proportion 0.253 0.494 0.253  ## Base Risk Model A simplified covariate-adjusted risk model as developed by Steyerberg is fitted below. For simplicity when later interacting baseline variables with treatment, the important age by Killip class interaction is omitted. Pulse rate is modeled using a linear spline with a knot at 50 beats/minute. Code require(rms) options(prType='html') dd <- datadist(gusto); options(datadist='dd') f <- lrm(day30 ~ tx + age + Killip + pmin(sysbp, 120) + lsp(pulse, 50) + pmi + miloc, data=gusto, eps=0.005, maxit=30) f Logistic Regression Model lrm(formula = day30 ~ tx + age + Killip + pmin(sysbp, 120) + lsp(pulse, 50) + pmi + miloc, data = gusto, eps = 0.005, maxit = 30)  Model Likelihood Ratio Test Discrimination Indexes Rank Discrim. Indexes Obs 40830 LR χ2 4143.09 R2 0.243 C 0.820 0 37979 d.f. 12 R212,40830 0.096 Dxy 0.641 1 2851 Pr(>χ2) <0.0001 R212,7955.8 0.405 γ 0.641 max |∂log L/∂β| 4×10-9 Brier 0.055 τa 0.083 β S.E. Wald Z Pr(>|Z|) Intercept -3.4692 0.7095 -4.89 <0.0001 tx=SK 0.0742 0.0513 1.45 0.1483 tx=tPA -0.1358 0.0609 -2.23 0.0258 age 0.0796 0.0021 37.12 <0.0001 Killip=II 0.6011 0.0511 11.76 <0.0001 Killip=III 1.2092 0.1053 11.49 <0.0001 Killip=IV 1.9292 0.1391 13.87 <0.0001 sysbp -0.0391 0.0017 -23.40 <0.0001 pulse -0.0210 0.0141 -1.48 0.1376 pulse' 0.0406 0.0144 2.82 0.0048 pmi=yes 0.4714 0.0486 9.70 <0.0001 miloc=Other 0.3144 0.1162 2.70 0.0068 miloc=Anterior 0.5441 0.0444 12.27 <0.0001 ## Semi-Saturated Model The only way that ORs for treatment can vary in a logistic model is for there to be one or more interactions between baseline covariates and treatment. Here we allow for all interactions with treatment, which will allow ORs, RRs, and ARRs to vary as the data dictate, assuming that three-way interactions are not needed, e.g., the infarct location-specific treatment effect does not depend on age. Code g <- lrm(day30 ~ tx * (age + Killip + pmin(sysbp, 120) + lsp(pulse, 50) + pmi + miloc), data=gusto, eps=0.005, maxit=60) print(g, coefs=FALSE) Logistic Regression Model lrm(formula = day30 ~ tx * (age + Killip + pmin(sysbp, 120) + lsp(pulse, 50) + pmi + miloc), data = gusto, eps = 0.005, maxit = 60)  Model Likelihood Ratio Test Discrimination Indexes Rank Discrim. Indexes Obs 40830 LR χ2 4159.64 R2 0.244 C 0.821 0 37979 d.f. 32 R232,40830 0.096 Dxy 0.641 1 2851 Pr(>χ2) <0.0001 R232,7955.8 0.405 γ 0.642 max |∂log L/∂β| 1×10-10 Brier 0.055 τa 0.083 The likelihood ratio test is the gold-standard frequentist method for testing for added value of the treatment interactions, i.e., whether the treatment ORs are constant over covariate settings. By using a chunk test to test whether any of the baseline variables interact with treatment, this method has a perfect multiplicity adjustment in the frequentist sense. Code lrtest(f, g)  Model 1: day30 ~ tx + age + Killip + pmin(sysbp, 120) + lsp(pulse, 50) + pmi + miloc Model 2: day30 ~ tx * (age + Killip + pmin(sysbp, 120) + lsp(pulse, 50) + pmi + miloc) L.R. Chisq d.f. P 16.552157 20.000000 0.681833  We see no evidence to suggest HTE for t-PA or SK effects. We can also use AIC to assess whether allowing for interactions will likely result in better patient-specific outcome predictions. Code c(AIC(f), AIC(g)) [1] 16558.31 16581.76 The simpler model has a lower (better) AIC, indicating that treatment interactions are not strong enough to overcome the overfitting that allowing for them would entail. In other words, the interactions are likely more noise than signal. We can also compare the two models by computing the relative explained variation on the risk scale as detailed here. Code rexv <- var(predict(f, type='fitted')) / var(predict(g, type='fitted')) rexv [1] 0.9959584 From this we see that even without penalizing for overfitting, all the treatment interactions account for only 0.004 of the predictive information. The no-interaction logit-additive model that assumes constancy of treatment ORs is at least 0.996 adequate on a scale from 0 to 1. ## Back to the Difficulty of the Task Covariate-specific treatment effects are combinations of main effects and interaction effects, and one needs evidence that the interaction effect is non-zero before covariate-specific relative efficacy is interesting. As an example of how difficult it is to estimate differential treatment effects (here, double differences on the logit scale, or ratios of odds ratios), let’s temporarily re-fit the logistic model including only a single treatment interaction—with location of infarct. Consider just inferior vs. anterior infarcts, which are the dominant categories and are not too imbalanced. We estimate the double difference in log odds for t-PA vs. SK in anterior infarcts minus t-PA vs. SK in inferior infarcts. Then we get the frequency-weighted overall treatment effect in these two groups, which is similar to dropping the interaction from the model. Code i <- lrm(day30 ~ tx * miloc + age + Killip + pmin(sysbp, 120) + lsp(pulse, 50) + pmi, data=gusto, eps=0.005, maxit=30) contrast(i, list(tx='tPA', miloc='Inferior'), list(tx='SK', miloc='Inferior'), list(tx='tPA', miloc='Anterior'), list(tx='SK', miloc='Anterior'))  age Killip sysbp pulse pmi Contrast S.E. Lower Upper Z 1 61.516 I 130 73 no 0.1546134 0.1081337 -0.05732489 0.3665516 1.43 Pr(>|z|) 1 0.1528 Confidence intervals are 0.95 individual intervals Code w <- with(gusto, c(sum(miloc=='Inferior'), sum(miloc=='Anterior'))) contrast(i, list(tx='tPA', miloc=c('Inferior', 'Anterior')), list(tx='SK', miloc=c('Inferior', 'Anterior')), type='average', weights=w)  Contrast S.E. Lower Upper Z Pr(>|z|) var 1 -0.1838079 0.05588118 -0.293333 -0.07428276 -3.29 0.001 0.003122706 Confidence intervals are 0.95 individual intervals The first contrast (double difference; differential treatment effect) has a standard error that is twice that of the second contrast (treatment effect averaged over the two infarct locations), and only the latter overall treatment effect has evidence for being non-zero (i.e., a benefit of t-PA). ## Penalization Bayesian modeling of HTE uses hierarchical random effects with shrinkage of interaction effects towards zero, e.g., shrinkage of “subgroup” effects towards the grand mean treatment effect. Borrowing information in this fashion is an optimal way to get covariate-specific treatment effect estimates. The frequentist counterpart that handles mixtures of continuous and categorical baseline variables is penalized maximum likelihood estimation. We will leave the main effects unpenalized, favoring an additive (in the log odds) model with many fewer parameters but allowing the completely unpenalized model to also have a chance at winning, and find the penalty for interaction terms that optimizes the effective AIC, i.e., the AIC computed using the effective degrees of freedom. Note that the pentrace function uses the likelihood ratio χ2 scale for AIC unlike the traditional AIC formula, i.e., here bigger AIC is better. Code h <- update(g, x=TRUE, y=TRUE) p <- pentrace(h, list(simple=0, interaction=1000* c(1, 5, 10, 15, 20, 30, 40, 100, 200, 500, 1000)), maxit=30) p  Best penalty: simple interaction df 0 1e+06 12.03211 simple interaction df aic bic aic.c 0 1000 20.33720 4111.002 3935.753 4110.981 0 5000 15.75213 4115.700 3979.961 4115.687 0 10000 14.32236 4117.036 3993.618 4117.025 0 15000 13.69510 4117.613 3999.600 4117.603 0 20000 13.33778 4117.937 4003.003 4117.928 0 30000 12.94300 4118.289 4006.757 4118.280 0 40000 12.72881 4118.477 4008.791 4118.469 0 100000 12.30905 4118.837 4012.768 4118.829 0 200000 12.15779 4118.963 4014.197 4118.955 0 500000 12.06394 4119.040 4015.083 4119.032 0 1000000 12.03211 4119.066 4015.383 4119.058 Code h <- update(g, penalty=list(simple=0, interaction=30000), maxit=30, eps=0.005) effective.df(h)  Original and Effective Degrees of Freedom Original Penalized All 32 12.94 Simple Terms 11 11.00 Interaction or Nonlinear 21 1.94 Nonlinear 3 1.05 Interaction 20 0.94 Nonlinear Interaction 2 0.05 The following plots show first the main effect coefficients (with interaction effects penalized vs. not penalized), and second the interaction effect coefficients. Note that main effect coefficients can change just because of penalizing interactions, just as removing interactions from models can greatly change main effects. Code ia <- grep('\\*', names(coef(g))) x <- coef(g)[-c(1,ia)]; y <- coef(h)[-c(1,ia)] r <- range(c(x, y)) plot(x, y, xlab='Unpenalized Coefficients', ylab='Penalized Coefficients', main='Main Effects', xlim=r, ylim=r) abline(a=0, b=1, col=gray(.85)) Code x <- coef(g)[ia]; y <- coef(h)[ia] r <- range(c(x, y)) plot(x, y, xlab='Unpenalized Coefficients', ylab='Penalized Coefficients', main='Interaction Parameters', xlim=r, ylim=r) abline(a=0, b=1, col=gray(.85)) Of the penalties studied, the best penalty by both AIC and corrected AIC is 10^{6} which effectively makes the model estimate 12.03 parameters instead of the original 12 parameters. The optimum penalty is really infinity, consistent the the likelihood ratio test for presence of any interactions with treatment. To give the benefit of the doubt we will use a penalty of 30000 to achieve an effective d.f. for interactions of 0.94. ### Using the Penalized Semi-Saturated Model to Estimate Distributions of Various Effects Even though there is no statistical evidence for HTE (i.e., interactions with treatment on an appropriate scale), we will still allow for all interactions, to give the benefit of the doubt. But we are penalizing the interaction parameters closer to what will cross-validate. Let’s start with what I believe is the best way to communicate results to an individual patient: estimating the absolute risk of mortality under two treatment options, without hinting how the patient should summarize the two estimates (absolute vs. absolute difference, etc.). The baseline covariate values for one patient are listed below. Code d <- data.frame(tx=c('SK', 'tPA'), Killip='II', age=55, pulse=40, sysbp=100, miloc='Inferior', pmi='no') p <- predict(h, d, type='fitted') names(p) <- c('SK', 't-PA') p  SK t-PA 0.04043122 0.03302851  Now turn to estimating treatment effects for all the patients in the RCT. To estimate patient-specific treatment effects on various scales, we estimate risks of dying within 30 days for each patient with their own covariate settings (baseline covariate values). This is done first with treatment set to SK, no matter which treatment the patient actually received. Then predictions are done setting treatment to t-PA. Code d <- gusto d$tx <- 'SK'
psk  <- predict(h, d, type='fitted')
d\$tx <- 'tPA'
ptpa <- predict(h, d, type='fitted')
k  <- c(5,10,15,20,50,100,200,400,800,1600,3200,20000)
pl <- c(0, quantile(psk, 0.99))
j  <- pmax(psk, ptpa) <= pl
co <- gray.colors(10, 0.75, 0)
ggfreqScatter(psk[j], ptpa[j], cuts=k, fcolors=co,
xlab='P(death|SK)', ylab='P(death|t-PA)')

Here is the distribution of estimated ARR across all patient types.

Code
dif <- psk - ptpa
s   <- c(mean=mean(dif), median=median(dif))
s
       mean      median
0.011224041 0.006971595 
Code
histSpike(dif, bins=seq(-0.02, 0.1, by=0.001), xlim=c(-0.02, 0.1),
xlab='ARR with t-PA', minimal=TRUE)
arrows(s, rep(-0.17, 2), s, rep(-.05, 2), xpd=NA, length=.05) # -0.04 -0.01 for full size

And the distribution of estimated RR.

Code
histSpike(ptpa / psk, xlim=c(.75, 1), minimal=TRUE,
xlab='RR t-PA/SK')

And finally the distribution of estimated ORs.

Code
or <- exp(qlogis(ptpa) - qlogis(psk))
histSpike(or, xlim=c(.75, 1), minimal=TRUE, xlab='OR')

The following scatterplot shows the relationship between estimated RRs and estimate ORs.

Code
ggfreqScatter(or, ptpa / psk, cuts=k, xlab='OR', ylab='RR', fcolors=co)

See that the variation in absolute or relative risk reduction is large, but adjusted odds ratios for t-PA vs. SK have a very narrow distribution. If we relied on the formal interaction test, this distribution would have a single value.

To see how the various measures depend on baseline risk, see the following graphs.

Code
xl <- 'Risk Under Control Treatment (SK)'
j <- psk <= pl
ggfreqScatter(psk[j], psk[j] - ptpa[j], cuts=k, xlab=xl, ylab='ARR', fcolors=co)
Code
ggfreqScatter(psk[j], ptpa[j] / psk[j], cuts=k, xlab=xl, ylab='RR', fcolors=co)
Code
ggfreqScatter(psk[j], or[j], cuts=k, xlab=xl, ylab='OR', fcolors=co)